Potassium Transport in Suspension Culture Cells and Protoplasts of Carrot

Potassium transport in suspension culture cells and protoplasts of carrot.

The properties of potassium transport in carrot (Daucus carota L.) suspension culture cells and their isolated protoplasts were examined. Cells cultured in Murashige and Skoog (MS) medium (Plant Physiol 15: 473-497) were potassium saturated and, consequently, they exhibited little net potassium accumulation. Cells that transport and accumulate potassium were derived from the MS-grown cells by c...

Carrot Suspension Culture Cells

Synchronization of somatic embryogenesis in a carrot cell suspension culture.

Synchronization of somatic embryogenesis was achieved in a carrot (Daucus carota L. cv. "Kurodagosun") suspension culture by sieving the initial heterogeneous cell population, by density gradient centrifugation in Ficoll solutions, and by subsequent repeated centrifugations at a low speed (50g) for a short time (5 seconds), followed by transferring the cell clusters obtained, which were compose...

Regulation of the shikimate pathway of carrot cells in suspension culture.

The activity of the first enzyme of the shikimate pathway, 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase, is demonstrated in extracts of Daucus carota cells grown in suspension culture. Maximum specific enzyme activity is found midway through the logarithmic growth of the culture; cells in lag and stationary phases of growth have lower enzyme levels. The enzyme is activated by tyrosine a...

Ion Transport in Isolated Protoplasts from Tobacco Suspension Cells: II. Selectivity and Kinetics.

Protoplasts were enzymically isolated from suspension cultured cells of Nicotiana glutinosa L. and aspects of transport selectivity and kinetics were studied. In the presence of Ca(2+), transport was selective for K(+) ((86)Rb) over Na(+). (36)Cl(-) transport was inhibited by Br(-) or I(-) but not by H(2)PO(4) (-). The kinetic data for short term (30 minutes) K(+) influx over the range of 0.05 ...